1. Structure and ionization properties of amino acids and polypeptides. Ionization equations and calculation of the net charge of polypeptides. 2. Secondary and tertiary structures of proteins, the Ramachandran angles(dihedral angles) of peptides.Secondary and Tertiary structures of proteins 3. Molecular Recognition concept: receptors ligand interactions, enzyme-substrate interactions. The Scatchard equation. Proximity and orientation effects, induced-fit concepts in enzyme-substrate molecular interactions. 4. Importance of enzyme inhibitors and applications in modern drug developments. Derivation of the equations: Michaelis-Menten equation, equation for the kinetics of competitive enzyme inhibitors. 5. Chromatograhic techniques for biochemical research: ion-exchange, gel- filtration, affinity chromatography, HPLC. 6. Enzyme catalytic principles and mechanisms: Serine proteases, Aconitase, Carboxypeptidase, Coenzyme and mechanisms 7. DNA structure, major groove and minor groove figures. DNA-protein interactions, DNA-small ligand interactions. Restriction endonuclease mechanisms.

Major journals: PNAS, Nature, Science, JACS, J.Biol.Chem.