114年第1學期-5369 結構生物化學 課程資訊

評分方式

評分項目	配分比例	說明
mid term and term examinations	50

教育目標

1. Structure and ionization properties of amino acids and polypeptides. Ionization equations and calculation of the net charge of polypeptides. 2. Secondary and tertiary structures of proteins, the Ramachandran angles(dihedral angles) of peptides.Secondary and Tertiary structures of proteins 3. Molecular Recognition concept: receptors ligand interactions, enzyme-substrate interactions. The Scatchard equation. Proximity and orientation effects, induced-fit concepts in enzyme-substrate molecular interactions. 4. Importance of enzyme inhibitors and applications in modern drug developments. Derivation of the equations: Michaelis-Menten equation, equation for the kinetics of competitive enzyme inhibitors. 5. Chromatograhic techniques for biochemical research: ion-exchange, gel- filtration, affinity chromatography, HPLC. 6. Enzyme catalytic principles and mechanisms: Serine proteases, Aconitase, Carboxypeptidase, Coenzyme and mechanisms 7. DNA structure, major groove and minor groove figures. DNA-protein interactions, DNA-small ligand interactions. Restriction endonuclease mechanisms.

基本資料

選修課，學分數：2-0
上課時間：四/3,4[CH012]
修課班級：化學系4,碩博1,2
修課年級：1年級以上
選課備註：大四可選。

參考書目

Major journals: PNAS, Nature, Science, JACS, J.Biol.Chem.