本課程先以引導學生瞭解分離技術於各式生物樣品製備之應用，隨後再介紹以層析理論為基礎之各式化學分離方法。

1. 加強學生在分離化學方面的知識，以及如何使用分離技術來純化/分離化合物。 2. 找出各種分離/萃取技術的差異和實用性，以及如何選擇合適的樣品前處理方法來萃取特定基質中的目標分析物。 1. Enhance students’ knowledge in separation science, and how to perform separation techniques to purify/isolate compounds. 2. Figure out the differences and practical in various separation/extraction techniques, and how to select an appropriate sample pretreatment approach to extract target analytes in specific matrix.

1. John R. Dean, Extraction Techniques in Analytical Sciences, John Wiley & Sons, Inc., 2010. (e-book is available)

2. Elena Ibanez and Alejandro Cifuentes, Green Extraction Techniques: Principles, Advances and Applications (eBook ISBN: 9780444638694). Elsevier Science Serials, 2017

3. Wenkui Li, Wenying Jian, Yunlin Fu, Sample Preparation in LC-MS Bioanalysis (ISBN: 978-1119274292), John Wiley & Sons, Inc., 2019.

4. Somenath Mitra, Sample Preparation Techniques in Analytical Chemistry, John Wiley & Sons, Inc., 2003. (e-book is available)

5. Douglas E. Raynie, Modern Extraction Techniques, Anal. Chem. 2010, 82, 4911–4916.